Genetic Engineering
Protein Hydrolysates in Fermentation Reactions with Genetically Engineered Micro-organisms
With the advent of genetic engineering, the use of fermentation processes sharply increased. In 1977, Blattner reported pioneering work on the use of Charon phages as vectors for cloning DNA in Escherichia coli. In this and subsequent research, Blattner used N-Z-Amine™ A, Sheffield’s enzymatically-hydrolysed casein, in media designed to enable the optimal growth of E. coli.
Because of the safety and applicability of these methods, both the National Institutes of Health and the Cold Spring Harbor Laboratory have used media containing N-Z-Amine™ A and recommended them in their publications.
Since then, N-Z-Amine™ A and other Sheffield™ hydrolysed proteins as Hy-Case™ M have been utilized in many media formulations as economical, yet highly active and pure ingredients in genetic research. As these studies evolve into commercial applications, the consistent quality and availability in bulk quantity of these products will make them all the more attractive.
Sheffield™ Pharma Ingredients continues to expand the range of Sheffield™ brand products and today offers over 50 different hydrolysed proteins on basis of various protein raw materials and enzymes to enable both highly specific and bulk fermentation reactions