Sheffield rInsulin CC
Insulin is a hormone that is important in the regulation of carbohydrate and fat metabolism in mammals. It is no surprise; therefore, that insulin also has an application in mammalian cell culture. Insulin in-vitro acts as a mitogen for most cells and is involved in glucose and lipid metabolism, amino acid uptake and DNA synthesis. The supplementation of cell culture media with insulin stimulates growth and proliferation of a variety of somatic cells. The mechanism of mitogenic action of insulin tends to be cell-line dependent. Insulin stimulates cell growth by binding to glycoprotein receptors on the cell surface. Most studies have found that insulin exerts it’s mitogenic effects at 1-20 µg/ml and therefore 1-20 mg/L. In CHO cells insulin has pulled cells back from the verge of apoptosis by increasing the metabolism of glucose and thus decreasing reactive oxygen species. Insulin has become a necessary supplement in many serum free media formulations. Insulin is suitable for most industrial relevant cell lines and is present in many commercial ‘protein-free’ media.
Sheffield rInsulin CC, is animal origin free (AOF) in the first degree, otherwise known as Primary AOF, as it's components are not directly derived from eukaryotic animals. This product should be of interest to those industrial biopharma producers who do not require, or are uninterested to pay a premium for, Tertiary AOF (a.k.a. AOF in the 3rd degree) rInsulin.
Sheffield rInsulin CC is recommended for use as a cell culture supplement. Recommended dosage is approximately 2–20 mg/L in media, but should be optimized on a process basis.
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